• Antigen preperation and animal immunization: proteins, peptides, cells, DNAs etc. and in vitro immunization.
• Conventional techniques of hybridoma production: generation of hybridomas from spleen cells of immunized mice, rats, and rabbits.
• Hybridoma expansion and subcloning.
• Cryopreservation of hybridomas secreting mAbs.
• Monoclonal antibody isotyping.
• ELSIA screening of hybridoma supernatants and mAb characterization by immunoprecipitation, western blot, immunohistochemistry, immunofluorescence staining, FACS, in vitro bio-assays, and affinity measurements.
• Production of mAbs - high density cell culture techniques.
• Purification of mAbs - FPLC affinity columns.
• Generation of bi-specific mAbs by secondary fusion.

CONTACT:

Mr. Tom DeKoning, Marketing Manager, CTA Corp CTACorp@vai.org

Brian Cao, M.D., Senior Scientific Investigator, MATCbrian.cao@vai.org